So, this is my first time using Mothur on Galaxy. I am following the protocol https://galaxyproject.github.io/training-material/topics/metagenomics/tutorials/mothur-miseq-sop/tutorial.html
I am currently stuck at the 3rd step in the process (quality control)
when I make it to the make.contig section the program will not use my paired reads from the previous step, it keeps defaulting to a pair from the Un-paired data and giving me the error message:
Fatal error: Exit code 127 () mothur: error while loading shared libraries: libboost_iostreams.so.1.57.0: cannot open shared object file: No such file or directory mothur: error while loading shared libraries: libboost_iostreams.so.1.57.0: cannot open sh
I feel as if I am missing a step. If someone could run through the tutorial and see if it truly is a bug or if it is something i am doing it would be greatly appreciated.
Where are you executing the tutorial? Galaxy main (https://usegalaxy.org) or ??
I'm executing the tutorial in the main Galaxy page using the NGS: Mothur tool set.