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• How to split a merge piared data using ncbi SRA tools?
  can I split a merged paired end extract reads into two separate data (acc. to their ends) using n...
• Can someone give me a foolproof instruction on how to merge R1 and R2 illumina fastq files
  Dear Galaxy experts,               I need to merge R1 and R2 Illumina paired end reads. T...
• Merging BAM files: bamtools not found
  I have BAM files from aligning paired end Illumina reads. I would like to merge the aligned seque...
• miSEQ data analysis
  I have the miSeq paired end reads as two different fastaq files. How can I assemble/merge them th...
• Freebayes issue on merged data
  Hi, I am completely new to this field so I'm hoping this is a simple mistake on my part. I'm tr...
• Correct way of merging samples for father, mother, child trio variant calling
  I am new to NGS data analysis and I'm working in a multiple-sample variant calling workflow. I ha...
• Fastq Quality Trimmer For Pe Illumina?
  Hello, I was wondering, can Galaxy's FASTQ Quality Trimmer tool be used on Illumina paired-end d...
• PEAR alternative for merging overlapping paired-end reads using Galaxy tools
  Hello! I typically use PEAR to merge my two overlapping paired-end reads for one high quality re...
• trimmomatic with paired-end
  Dear Biostar, I have paired-end sequencing data I would like to trim using trimmomatic. I have r...
• cuffmerge for single end and paired end
  is it correct to merge the single end and paired end data together in cuffmerge? if yes then why ...
• why "PEAR - Paired-End reAd mergeR" this tool disappear from Galaxy?
  Hello, does everyone know why "PEAR - Paired-End reAd mergeR" this tool disappear from Galaxy? Be...
• Pre-Processing Of Illumina Rna-Seq Paired End Data
  Hello, I have Illumina 76bp paired end data for a zebrafish RNA-seq experiment and am basically ...
• Making paired-end reads the same length
  I have paired-end data (in two separate files).  I have groomed my files, and would like to filte...
• Should Fastq Files Be Merged?
  Hi, I have downloaded the fastq.tgz files for an ENCODE RNA-SEQ data and unpacked the files. The...
• Which Galaxy tool can concatenate fastq files from different lanes of flow-cell?
  Which tool on Galaxy can I use to merge my fastq files from lanes 1,2,3, and 4 of a single librar...
• Regarding splitter or merging the paired end sequencing file
  hello, Recently i got paired end data and firstly, i removed the adopter sequences. For that i u...
• Analysis of paired end illumina sequence.
  Hi everyone, I am  new in NGS.  I would like to filter and identify V4 region from raw paired en...
• Merging Fastq Files
  Hi I have received Illumina paired-end genome sequence data as a .tar file. When unpacked the dat...
• Nextera pair end adapter cleaning
  Can I use Nextera pair end adapter option in Galaxy Trimmomatic program for cleaning my illumina ...
• Tophat Paired End Read
  Hi all, I have paired end RNA-Seq reads ( Illumina Hiseq 2000) in seperate files. Before mapping...
• Splitting Biased Paired End FASTQ data
  The FASTQ splitter in Galaxy warns that the tool only works if Paired-end data has equal length r...
• Do I trim paired end sets first or merge and then trim?
  Hi there, I have a quick question - I have raw 10Gb -sized datasets for my samples. For each one...
• Trimming And Thinning Of Paired-End Reads
  Hi galaxy users, I've been experiencing some problems trimming the adapters and filtering by qua...