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Question: Cuffdiff problems as part of a tutorial
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gravatar for k.l.noel
9 months ago by
k.l.noel • 0
k.l.noel • 0 wrote:

Hello,

I have a problem with Cuffdiff, having successfully executed Tophat, Cufflink and Cuffmerge on RNAseq data as part of a tutorial. Here is the error message I received.

Fatal error: Exit code 1 () Error: sort order of reads in BAMs must be the same

I tried to run Cuffdiff twice and it did not work.

Any suggestions would be appreciated.

Thanks,

Katie
rna-seq gtf genome sort cuffdiff • 411 views
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modified 9 months ago by Jennifer Hillman Jackson ♦ 25k • written 9 months ago by k.l.noel • 0
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gravatar for Jennifer Hillman Jackson
9 months ago by
Jennifer Hillman Jackson ♦ 25k
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

The error is coming up because some of the Tophat jobs were mapped against papHam1 (the default in the target database/genome select list on the tool form) instead of actively selected to be hg19.

Rerun: the Tophat jobs to use hg19 instead, then the derivative Cufflinks, Cuffmerge, and Cuffdiff jobs.

Everything else in your procedure looks great!

Thanks! Jen, Galaxy team
ADD COMMENT • link written 9 months ago by Jennifer Hillman Jackson ♦ 25k
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