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• Query Regarding Rna-Seq Data Analysis
  Hi, I am having a fastq file for my RNA data analysis in my computer. How to make it available f...
• Where is "Tophat for illumina Find splice junctions"?
  Dear Sir/Madam: This is Bob. I am interested in the course of RNA-seq-analysis-mapping. The demo...
• Tophat error: An error occurred with this dataset
  Hi, I am new to RNA-seq data analysis and want to identify differentially expressed genes from ...
• Need help with "Tophat for Illumina" tool
  I do not know why: "RNA-Seq FASTQ file:" can not been selected?      Tool name: Tophat for Il...
• RNA-seq data analysis workflow!
  Hello, I am a newcomer to galaxy based data analysis.I am looking for a workflow to analyse RNA-...
• HG38 .gtf file
  Hello. For practise, I am running an RNA-seq analysis on some of the RNA-seq data from Illumina ...
• TopHat with Culex quinquefasciatus genome
  I try to use TopHat for RNA-seq data. The Culex quinquefasciatus genome will be used for the TopH...
• Cloudman - Galaxy - TopHat2 error
  Hi all, I'm going through the RNAseq tutorial posted here: https://usegalaxy.org/u/jeremy/p/gal...
• Mapping With Tophat Vs. Bwa
  Hello, I am getting what seems to me to be strange results using two different mapping tools in ...
• Importing Reference Genome Into Galaxy For Tophat2
  Hi, I am new to the field of RNA-Seq analysis. I'd be very greatful if somene could help me with ...
• TopHat RNA-Seq FASTQ file not seen
  Hey, I'm trying to use Galaxy to process my RNA-seq data. I have uploaded the fastq file to gal...
• Tophat Issues
  To whom it may concern I do have a problem with tophat. I can easily put fastq data to "history"...
• Analysis workflow of miRNA-seq data
  I'm looking to analyse RNA-Seq data from miRNA. How similar is the workflow to RNA-seq analysis?
• unable to load zebrafish GTF/GFF3 files from current history into any of the RNA analysis tools
  I am analyzing zebrafish RNA seq data, I am able to upload the fastq files, use trimmomatic, alig...
• Finding gene names from TopHat results.
  Hello, I am trying a build a workflow for RNA-Seq analysis and I want to verify my methods via co...
• TopHat align summary
  Hello, I am using galaxy to analyze RNA seq of 100bp sing end data, sequenced with Illumina 2500...
• Pre-Processing Of Illumina Rna-Seq Paired End Data
  Hello, I have Illumina 76bp paired end data for a zebrafish RNA-seq experiment and am basically ...
• TopHat alignment of RNA seq data
  Hello everyone, I want to use TopHat trough Galaxy to perform the alignment of single end RNA Se...
• What is the sequence of Illumina Universal Adapters?
  I am currently analyzing RNA-seq data. I would anyways be trimming the adapters. However, it woul...
• Tophat error "long_spanning_reads"
  Hello. I have ran Tophat for Illumina with groomed RNA-seq data and it is marked red and has an ...
• Denovo with tophat
  Hello everyone. I am new to RNA Seq and need help. I just want to ask if it is right to do de no...