Question: Tophat failure and calling for error in setting metadata
0
gravatar for anchalkar.kaushal2007
23 months ago by
anchalkar.kaushal200740 wrote:

Hello, Tophat is consistent in showing errors and does not give proper results to further the workflow for cuifflink. Kindly look into the matter. the tophat step isn't completed and continuously shows the same error

Thank you

Regards Kaushal India

rna-seq tophat breast cancer • 432 views
ADD COMMENTlink modified 23 months ago by Jennifer Hillman Jackson25k • written 23 months ago by anchalkar.kaushal200740
0
gravatar for Jennifer Hillman Jackson
23 months ago by
United States
Jennifer Hillman Jackson25k wrote:

Hello,

Please see this reply for a description of the known issue: https://biostar.usegalaxy.org/p/20939/

The way to work around this is to run analysis through Tophat, fix the metadata, then proceed with downstream tools. If using a workflow, this means that it should be split into two - one that runs tools through Tophat, and one that runs tools after Tophat. In between the two, correct the metadata as described in the linked post above.

Thanks, Jen, Galaxy team

ADD COMMENTlink written 23 months ago by Jennifer Hillman Jackson25k
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