Hello,
I have just aligned some reads using Bowtie2 and am wanting to look at correlation between my replicates using the plotCorrelation tool. However, this requires a matrix file to first be generated with the multiBamSummary tool. I tried running the latter to generate the matrix but this error message came up:
Fatal error: Exit code 1 () Traceback (most recent call last): File "/galaxy-repl/main/jobdir/014/048/14048548/conda-env/bin/multiBamSummary", line 7, in <module> main() File "/galaxy-repl/main/jobdir/014/048/14048548/conda-env/lib/python2.7/site-packages/deeptools/multiBamSummary.py", line 224, in main num_reads_per_bin = c.run(allArgs=args) File "/galaxy-repl/main/jobdir/014/048/14048548/conda-env/lib/python2.7/site-packages/deeptools/countReadsPerBin.py", line 263, in run raise ValueError("numberOfSamples has to be bigger than {} ".format(min_num_of_samples)) ValueError: numberOfSamples has to be bigger than 99567
Could anyone help this ?
Cheers, Alfred
Which reference genome did you use?