Question: Another step to make Trinity output usable with Blast
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gravatar for RACHAMPION
2.1 years ago by
RACHAMPION • 10
RACHAMPION • 10 wrote:

I have an output from Trinity that looks like an assembled genome. When I use Blast to check for similarity, I get the message:

Your BLAST job specified more than one input sequence. This box lets you choose which input sequence to show BLAST results for.

Is there something that I need to do to join the Trinity file into a single genome?

Thanks Rick Champion

trinity web-blast blast galaxy • 638 views
ADD COMMENT • link • modified 2.1 years ago by Jennifer Hillman Jackson ♦ 25k • written 2.1 years ago by RACHAMPION • 10

This is a third party web Blast search (not within Galaxy)? It may only accept one query sequence at a time. The target genome can contain multiple sequences. However, the service you are using may not allow you to load a custom target genome (most don't).

Please clarify if this does not apply.

Jen, Galaxy team

ADD REPLY • link written 2.1 years ago by Jennifer Hillman Jackson ♦ 25k
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gravatar for Jennifer Hillman Jackson
2.1 years ago by
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello, Hopefully you solved the problem, Jen

ADD COMMENT • link written 2.1 years ago by Jennifer Hillman Jackson ♦ 25k
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