Question: Questions On Directionality
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gravatar for Maness, Nicholas J
6.8 years ago by
Maness, Nicholas J • 10 wrote:
Hi there, I apologize if this is covered in documentation or help threads. searched and it seemed it was not. I have several illumina rna-seq data sets that "should" be directional. It seems the directionality is very good, based on the visualization. First question is; in the visualization window, are the reads color coded by direction, i.e. are orange one direction and blue the other? If so, it looks like my data is >95% directional. Similar question, is there a way to quantify directionality of the data set? BTW, the data is RNA-seq data from rhesus macaques and i'm using the rhesus macaque genome build from UCSC. thanks very much, Nick Maness Tulane University
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ADD COMMENT • link • modified 6.8 years ago by Jeremy Goecks • 2.2k • written 6.8 years ago by Maness, Nicholas J • 10
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gravatar for Jeremy Goecks
6.8 years ago by
Jeremy Goecks • 2.2k
Jeremy Goecks • 2.2k wrote:
Nick, Different colors in read data does indicate strandedness; hover over the track and click on the 'Edit Settings' icon (gear) to see/change the sense/anti-sense colors used. You can use the Filter SAM tool to filter for mapped strand. Good luck, J.
ADD COMMENT • link written 6.8 years ago by Jeremy Goecks • 2.2k
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