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• Removal of primers using Trimmomatic
  Hello, Trimmomatic has an illuminaClip feature which appears to be able to cut custom sequences ...
• Problem running Clustal 2.1 Multiple Sequences Alignment - an instance of 'std::bad_alloc'
  Dear All, I've been trying to analyze reads from a short transcript. The data I have was obtaine...
• Primer Contamination, Miranalyzer
  Hi Galaxy, Ive got 2 problems for you; 1) Ive got microRNA Illumina NGS data that I want to ana...
• Degenerate primer removal tool on Galaxy?
    Hi there, I am working on a next generation sequencing dataset and can't seem to find a tool ...
• How to interpret and control for effects of primer contamination?
  Hi all, I'm currently analysing some RNAseq samples which were amplified prior to library prep an...
• Fastq format to final format
  Hi I used the galaxy to remove sequence debris and sequencing primers, as well as exclude small ...
• Invalid quality scores
  I'm trying to demultiplex some older 454 sequences (fasta + qual) so that I have individual fastq...
• Paired end illumina RNAseq reads - running trinity with paired trimmed files and adding unpaired as single read?
  We have paired end Illumina HiSeq 4000 reads that we are working to remove adaptors and trim base...
• Issue With Saving 'Manipulate Fastq' In Workflow; And Request For Advice Dealing With Barcoded 454 Data
  Hi, I'm a new user, learning how to use Galaxy while I wait for my 454 results. So I'm not actua...
• how to find adaptor sequences
  Hi    How can i find the adaptor sequence in my reads.
• Output of FLASH can't be read by other tools?
  Hi, I have used FLASH to assemble some reads. They are still in FASTq format. However I can't ge...
• Permanently removed all data but showing using 28% of disk
  Permanently removed all data, including hidden, but it is still showing 28% of disk usage
• Any tools for separate unpaired reads in paired-end sequencing fastq files?
  Hi, I would like to know if there is any tool can do the following job? I have some data files ...