Hello,
I recently had the genome of the bacteria that I am working on sequenced by MicrobesNG. The problem is that I don't know how to convert the contig data into a full genome sequence. I tried using Unicycler on the galaxy website, but the run always comes back with a "failure preparing job" error.
I've uploaded the fasta file that MicrobesNG provided me with, which looks as follows:
>NODE_1_length_363942_cov_11.1177
CGGCATCGGCGTC...
>NODE_2_length_340362_cov_12.5053
ATATGCGACCTCGAA...
etc.
>NODE_126_length_128_cov_211
CCCCCCCCCC
for the settings in Unicycler, I've tried "single" end data, with dragging the fasta file to the "unpaired reads" location, and I also select this fasta file in the "long reads" section. Otherwise I haven't changed any of the settings and just pressed "execute".
Does anyone know what I am doing wrong and how to fix it? I don't know if the data that I have is in an unconventional format and does it therefore need some preprocessing before I can submit it to Unicycler?
Thank you