Question: TSS distrubtion for ChIP-Seq
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xiang-jiao.yang • 10 wrote:
I have been using ChIP-Seq and want to display peak distribution at the transcription start sites (TSS). Is there a MakeTSSDist tool in Galaxy main?
I have been using ChIP-Seq and want to display peak distribution at the transcription start sites (TSS). Is there a MakeTSSDist tool in Galaxy main?
It makes much more sense to show the signal distribution around the TSS, since peak distribution will rely heavily on the peak caller and its settings. The normal way to do this is to make bigWig files with bamCoverage or bamCompare, compute the coverage around the TSS with computeMatrix reference-point and then plot it with plotProfile or plotHeatmap.