I would ask about which reference cow genome should I use for RNAseq data analysis in Galaxy to find new splicing events. I have the problem that in BosTau6 progesterone receptor has 7 exons but in BosTau7 has 9 exons. In the NCBI genes database progesterone receptor has 8 exons. There is also the newest BosTau8. I am not sure which is the proper one. From the right choice depend my final results. I would like also to ask if there is a tool in Galaxy to visualize new splicing forms of different genes.
Robert