Question: HISAT2 incompatible with CuffDiff ?
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willtun1991 • 10 wrote:
Hey
I'm trying to compare the transcript expression levels between two conditions. I have put my data through HISAT2 on galaxy. However when I put the HISAT2 outputs from condition 1 and condition 2 into cuffdiff, no matter what parameters and references I use, the galaxy run fails every time I try and implement cuffdiff.
Any one else finding the same problem?
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modified 2.7 years ago
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Jennifer Hillman Jackson ♦ 25k
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2.7 years ago by
willtun1991 • 10
There is a current issue with Main that the team is investigating. This is most likely the reason for the job failures. More feedback soon. Thanks for your patience. Jen, Galaxy team
Ok. Where can I find this feedback message that tells me cuffdiff works again?
Our team will post back here directly in reply to inquiries about the cluster performance (including yours). This should come today or tomorrow.
I can let you know now that all nodes are/were not impacted as of late yesterday. Many users have had success with re-running jobs a few times. I also had this type of result while testing. (Same job, re-run, some result in failure, others succeed).
Thanks for your patience. The core group is at an important meeting through today.