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Question: Put header to bigwig file
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gravatar for jxmarkfernandez
2.8 years ago by
jxmarkfernandez • 0
jxmarkfernandez • 0 wrote:

Hello,

I am working with ChIPseq with Histone marks on chicken (Gal/Gal4). I generated a bigwig file using the "bamcompare" tool in deepTools2 and now I want to upload it in UCSC. Nevertheless, I should give a header to the bigwig to upload it in UCSC, which I cannot do as Bigwig files are binary, or at least that's what a fellow bioinformatitian told me.

Can anybody help me?

Thanks for your attention,

Marc
header chipseq galaxy bamcompare bigwig • 891 views
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modified 2.8 years ago by Jennifer Hillman Jackson ♦ 25k • written 2.8 years ago by jxmarkfernandez • 0
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gravatar for Jennifer Hillman Jackson
2.8 years ago by
Jennifer Hillman Jackson ♦ 25k
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

Bigwig files are never really "uploaded" to UCSC in entirety, rather portions of the data are sent from one server to another. A custom track line is added at the step when this transfer is first initiated. The help page at UCSC for the datatype describes the complete process line-command but the final step is the same whether the data host server is Galaxy or not: http://genome.ucsc.edu/goldenPath/help/bigWig.html

Thanks, Jen, Galaxy team

 
ADD COMMENT • link modified 2.8 years ago • written 2.8 years ago by Jennifer Hillman Jackson ♦ 25k
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