Question: Question: Problems with Filter pileup when performing Heteroplasmy tutorial
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gravatar for mfoulk
2.8 years ago by
mfoulk • 20
mfoulk • 20 wrote:

Hello Galaxy,

I am trying to do the Heteroplasmy tutorial from the tutorial page so that I can use it as an exersize for an introductory bioinformatics class I am teaching. Everything seems to work fine except for the filter pileup tool. After I generate the pileup of mapped reads I go to Filter pileup to filter out positions with low coverage the "select dataset" field says that there are no pileup datasets available even though I just made a pileup file. Additionally, when I view the pileup file there are only 5 column when there  should be 6. It looks like the "read bases" (column 5) is missing. Is this a known problem? I was planning to use this tutorial on Monday so any help would be greatly appreciated! Thanks in advance.

galaxy • 714 views
ADD COMMENT • link • modified 2.8 years ago by Jennifer Hillman Jackson ♦ 25k • written 2.8 years ago by mfoulk • 20
2
gravatar for mfoulk
2.8 years ago by
mfoulk • 20
mfoulk • 20 wrote:

Nevermind. I found the answer in another thread. For anyone else having this problem, apparently you have to manually change change the datatype to pileup in the attributes.

ADD COMMENT • link written 2.8 years ago by mfoulk • 20

This is correct - thanks for posting back the answer here. Jen, Galaxy team

ADD REPLY • link written 2.8 years ago by Jennifer Hillman Jackson ♦ 25k
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