Question: fetch taxonomic representation tool only returns a small subset of original
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gravatar for rebeccarosetm
2.9 years ago by
rebeccarosetm • 0 wrote:

I used the NCBI BLAST+ blastn in Galaxy and get a return of approximately the same number of hits as my reads (approx  50,000). However when i use the Fetch Taxonomic Representation tool, I only get back some of the hits (10% or so), not in the same order, and with the correct annotation. I do have to use the Convert tool on the megablast result file because the FTR tool will not recognize the gi numbers with the pipes in the names (so I change the pipes to tabs). Manually checking the file, this appears to not introduce any errors. Help is appreciated! Thanks!

galaxy • 775 views
ADD COMMENT • link • modified 2.9 years ago • written 2.9 years ago by rebeccarosetm • 0
0
gravatar for Jennifer Hillman Jackson
2.9 years ago by
United States
Jennifer Hillman Jackson ♦ 25k wrote:

Hello,

The Fetch Taxonomic rep tool at http://usegalaxy.org has been pre-loaded with annotation that is a match for the Genbank databases available in the Megablast tool.

If you wish to use this function successfully with other databases, the tool can be installed and used in a local. If that is where you are using it already (a local or cloud would be needed for BLAST+), then double check how the data the tool is accessing is populated. It could be missing divisions or it is outdated or a mismatch for the blast databases.

Thanks, Jen, Galaxy team

ADD COMMENT • link written 2.9 years ago by Jennifer Hillman Jackson ♦ 25k
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gravatar for rebeccarosetm
2.9 years ago by
rebeccarosetm • 0 wrote:

Thank you Jennifer. Is it possible to specify with the megabits tool to only record one hit per read?

ADD COMMENT • link written 2.9 years ago by rebeccarosetm • 0
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