Question: Merging two fastq files together
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yenaoh90 • 0 wrote:
Hi all,
I've recently received the .fastq files for my rna-seq experiment. According to the sequencing centre, "the samples were run across 2 lanes," and I have received 2 single read .fastq files for each sample.
1) Can somebody explain what it means by "run across 2 lanes"?
2) Does "concatenate datasets tail to head" combine the two files by "merging" the two files on top of each other, or by "connecting" file 1 and file 2? In my case, how would I go about combining the two files before moving onto alignment using Tophat?
Thank you in advance,
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modified 3.3 years ago
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Jennifer Hillman Jackson ♦ 25k
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3.3 years ago by
yenaoh90 • 0
