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• FASTQ trimmer trimmed 1 nt too much of sequence but right amount of quality
  Hi,   I added two fastq files (I had just changed the datatype to fastqcsanger) to FASTQ trimme...
• trimming adapter sequences. is it necessary?
  Hi, I am a beginner to chip-seq analysis using Galaxy. I have received my data and did fastqc qua...
• quality score length differs from sequence length
  I have a fastq file that tophat2 can't process because one of the reads seems to have a sequence ...
• How To Analyze Genes Differential Expression Using Galaxy? Thanks.
  Hi All, Now I have two  Paired-End samples data ( they are  Next Generation Sequence Data and fa...
• Considerations for trimming poor quality Illumina MiSeq paired end reads
  I have generated fastq files from Illumina MiSeq for bacterial genome sequencing. My reads are 2...
• paired-end sequencing or ChIA-PET
  I want to look at original ChIA-PET datasets (FASTQ). It's a kind of paired-end sequencing. The s...
• Illumina HiSeq reads and Fastq joiner
   Hello, I have paired-end reads (Illumina HiSeq) from a metagenome in two Fastq files. These non...
• Fastq joiner and sequencing reads previously submitted to a quality based trimming
  Hello! I have a couple of doubts about Fastq joiner: 1) on the Fastq joiner instructions I see ...
• Problem with FASTQ Quality trimmer by sliding window
  Hi, I´m using the FASTQ Quality trimmer by sliding window with a quality score of 25, and after r...
• FastQ sanger sequences -ends quality trimming
  Dear all , I'm very naive regarding bioinformatic tools. I need to trim my sanger sequences to re...
• Analysis 454 Quality Data
  Hi, I am starting off with 454 read data in an sff file. I would like to get quality statistics ...
• Need help with "FASTQ Quality Trimmer" tool
  I have uploaded my fastQ file but it is not showing in drop down list of fastQ-Quality Trimmer to...